COMPARISON OF CEFOXITIN DISC DIFFUSION TEST, OXACILLIN SCREEN AGAR, AND PCR FOR mecA GENE FOR DETECTION OF MRSA
نویسندگان
چکیده
منابع مشابه
Comparison of cefoxitin disc diffusion test, oxacillin screen agar, and PCR for mecA gene for detection of MRSA.
UNLABELLED Cefoxitin is a potent inducer of the mecA regulatory system. It is being recommended for detection of methicillin resistance in Staphylococcus aureus (MRSA) when using disk diffusion testing. The aim of our study was to evaluate the efficacy of cefoxitin disc diffusion test to characterize MRSA and compare it with oxacillin agar screening and detection of mecA gene by PCR. MATERIAL...
متن کاملDetection of Oxacillin Resistance in Staphylococcus aureus: Comparison of Phoenix Oxacillin and Cefoxitin MICs, MicroScan Oxacillin MIC, Oxacillin and Cefoxitin Disk Diffusion, and mecA Gene Detection
BACKGROUND. Phenotypic tests for detection of mecA containing staphylococci have been challenged. Cefoxitin (FOX) disk diffusion (DD) has been reported to more accurately predict mecA gene presence than oxacillin (OX) DD. The Phoenix instrument rapidly provides identification and susceptibility data and it is of interest to determine if an experimental panel containing OX and FOX could accurate...
متن کاملEvaluation of Different Phenotypic and Genotypic Methods for Detection of Methicillin Resistant Staphylococcus aureus (MRSA)
Background: Methicillin resistant Staphylococcus aureus (MRSA) has been emerged as a nosocomial and community acquired pathogen worldwide. There are many challenges for laboratory detection of MRSA. The aim of this study was to compare different phenotypic methods with PCR based method as a gold standard for detection of mecA gene. <b...
متن کامل[Phenotypic methods for detection of methicillin-resistant Staphylococcus aureus].
BACKGROUND Cefoxitin is a potent inducer of the mecA gene. It is currently as a screening recommended method for presumptive identification of isolates of methicillin resistant Staphylococcus aureus (MRSA). The aim of the study was to compare the sensitivity and specificity of the cefoxitin disc diffusion (30 μg) to oxacillin agar screening from detection of the mecA gene by PCR. METHODS Thre...
متن کاملAssessment of methicillin resistant Staphylococcus Aureus detection methods: analytical comparative study
Introduction The heterogeneous expression of methicillin resistance in Staphylococcus aureus (MRSA) affects the efficiency of tests available to detect it. The objective of this study was to assess four phenotypic tests used to detect MRSA. Methods This is an analytical comparative study conducted among sudanese patients during period from May 2012 to July 2014, Staphylococcus aureus strains ...
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ژورنال
عنوان ژورنال: Indian Journal of Medical Microbiology
سال: 2009
ISSN: 0255-0857
DOI: 10.1016/s0255-0857(21)01748-5